Major experiments 1. Enzyme kinetics: Determination of Km and Vmax of the enzyme amylase (purified amylase). 2. Extraction of cellulase from a suitable fungal culture and study of enzyme activity by DNSA method. 3. Immobilisation of yeast cells and study of invertase activity.4. Quantitative study of diurnal fluctuation in Titratable Acid Number (TAN) in a CAM plant. 5. Extraction and estimation of GOT and GPT from suitable plant material.6. Determine the Chl a/Chl b ratio in C3& C4 plants.

Minor experiment 1. Separation of organic acids by paper chromatogra-phy.2. Separation of sugars by paper chromatography. 3. A study of the enzyme polyphenol oxidase, from po-tato peels. 4. Solvent extraction of chlorophyll a/b, xanthophylls and study of absorption pattern.5. Estimation of the total nitrogen content of a plant us-ing Kjeldahl’s method