Description
Molecular Methods & Biotechnology: From Gene Cloning to Proteomics
Master the tools that are defining the future of biology.
In the rapidly evolving landscape of life sciences, proficiency in molecular techniques is no longer optional—it is essential. Molecular Methods & Biotechnology provides a systematic, deep-dive exploration of the core technologies driving modern genetic engineering, diagnostics, and therapeutic discovery.
This concise yet comprehensive guide breaks down complex laboratory protocols into clear, conceptual frameworks, making it an indispensable resource for advanced students and laboratory professionals.
What You Will Master:
• Genetic Engineering: A complete roadmap of Recombinant DNA technology, including transgenic plant and animal production and the ethics of reproductive vs. therapeutic cloning.
• Next-Gen Sequencing: From Sanger’s foundational methods to high-throughput pyrosequencing and shotgun cloning.
• Advanced Proteomics: Techniques for protein characterization, including MALDI-TOF, Mass Spectrometry, and Yeast Two-Hybrid systems.
• PCR & Mutagenesis: A masterclass in PCR variants (RT-PCR, Real-time PCR) and precision genome editing through site-directed mutagenesis.
• Gene Silencing: Understanding the mechanisms of RNAi, siRNA, and Ribozymes to control gene expression.
• Molecular Markers: Practical applications of RFLP, RAPD, and QTL in map-based cloning and marker-assisted selection.
Why Choose This Guide?
Designed for quick navigation and high retention, this book strips away the fluff to focus on the principles, methods, and real-world applications of biotechnology. Whether you are prepping for a qualifying exam or designing a new research protocol, this text serves as your technical North Star.
Unlock the secrets of the genome. Advance your research. Master the molecules of life.
Dr. Mohammed Tarique is a highly accomplished scientific researcher whose work spans molecular biology, parasitology, and nanomedicine. Over a research career of more than 15 years, he has made impactful contributions to the understanding of malaria biology, helicase mechanisms, cancer therapeutics, and emerging applications of nanotechnology in biomedicine.
He has authored over 35 peer-reviewed publications in high-impact journals,
covering a wide spectrum of topics including genome-wide analyses, advanced drug-delivery strategies, and innovative anticancer approaches.
Beyond journal publications, Dr. Tarique has authored and contributed to several books with leading international publishers such as Springer Nature, CRC Press, IIP and Geh Press. These works highlight cutting edge themes in nanomedicine, nanoparticle based tissue engineering, and novel therapeutic targets against Plasmodium falciparum, reflecting his commitment to translating fundamental science into meaningful therapeutic directions.
Dr. Tarique's excellence has been recognized through notable honors and fellowships, including the UGC Dr. D. S. Kothari Postdoctoral Fellowship (2018). He also successfully qualified the CSIR-UGC National Eligibility Test (NET) for university lectureship in India. As a Bentham Science Ambassador, he actively supports scientific outreach and scholarly engagement. In addition, he holds key leadership positions, serving as Chairman of Almanac Social Welfare and Director of the First Aid Council of India, demonstrating a strong dedication to community service and public impact.
An active contributor to the global scientific ecosystem, Dr. Tarique serves on editorial and review boards for multiple respected international journals across microbiology, biotechnology, pathology, and molecular imaging. His academic journey includes a Master's degree in Biotechnology from Jamia Hamdard, followed by doctoral and postdoctoral research with the Parasite Biology Group at the International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi. His extensive laboratory experience also includes work as a Research Fellow at AlIMS, New Delhi, and as a Dr. D. S. Kothari Postdoctoral Fellow at Jamia Millia Islamia University.
In addition to research and academia, Dr. Tarique has contributed to innovative patent work in areas such as diabetic neuropathy treatment, non-invasive glucose monitoring, and malaria stage detection further underscoring his focus on practical, translational solutions to real-world health challenges.
Widely respected as a mentor, reviewer, and editor, Dr. Mohammed Tarique is known for his dedication to nurturing young researchers and strengthening the standards of scientific publishing. His enduring commitment to global health particularly in combating parasitic diseases-along with his drive for biomedical innovation, reflects his broader aspiration: to convert scientific progress into tangible societal benefit. With a robust record of publications, books, patents, and leadership initiatives, Dr. Tarique continues to help shape the future of biomedical research and inspire the next generation of scientists