The study of plants, including their taxonomy, structure and qualities, as well as biochemical processes, the research of plant diseases and the impact of the environment on plant growth, are the focus of the biological field known as plant science. Ayurveda, Pharmacy, Biotechnology, Microbiology, Biochemistry, Environmental Science, Biophysics, Agriculture, horticulture and forestry are examples of applied sciences built on the concepts and discoveries of Plant Sciences. One of the most fascinating and significant groupings of living things on Earth is the plant kingdom. They supply food, influence our environment, and act as a conduit for energy into the biosphere. New instruments and approaches were developed to aid in the study of the finer structure of various plants and their components. The use of a microscope not only made visible a vast array of tiny species, but also the tiniest intricacies of their interior architecture. Numerous novel instruments and methods have been invented throughout the history of biology, including microscopy, histochemistry, electrophoretic techniques, spectroscopic techniques, centrifugation techniques, and chromatography techniques, among others.
This book ‘Advanced Techniques in Plant Sciences’ includes the information about all basic as well as advanced techniques like, Microscopy, Polarizing Microscopy; Transmission and Scanning electron microscopes -Confocal Microscopy, Phase Contrast Microscopy; Photomicrography- Digital imaging and Microscopic measurements. The Spectroscopic techniques contents Colorimeter, Spectroscopic techniques, Ultraviolet–visible spectroscop; Infrared spectroscopy, Nuclear magnetic resonance spectroscopy; Basic NMR techniques, NMR Imaging Principles, Electron Spin Resonance (ESR) Spectroscopy, Atomic Absorption Spectroscopy (AAS); Mass spectrometry, Flame photometer, etc. In Histochemical Studies various methods that includes proteins, carbohydrates, lipids and enzymes; how to prepare the micro slides and the different methods of preparing the slides which includes. Along with this you will also study about whole mounts. Also about sectioning of plant specimens, free hand, microtome, rotary microtome sectioning and types of microtome, methods of serial sectioning. Maceration, Gel Electrophoresis, PAGE, SDS–PAGE, Agarose gel electrophoresis; Isoelectric focusing and 2D Electrophoresis. Blotting Techniques- principle and techniques of southern, northern and western blotting techniques and Southwestern Blotting. Centrifugation techniques, Types of Centrifugation, Ultracentrifugation; Concept of Partition Coefficient; Chromatography, Techniques by chromatographic bed shape, Paper Chromatography, Double-way paper chromatography, Planar chromatography, Thin layer chromatography (TLC), High-performance liquid chromatography (HPLC)- Partition chromatography, Normal-phase chromatography, Displacement chromatography, Reversed-phase chromatography (RPC), Size-exclusion chromatography, Ion-exchange chromatography, Bioaffinity chromatography, Aqueous normal-phase chromatography, Isocratic flow and gradient elution; High-Performance Thin-Layer Chromatography (HPTLC), Gas chromatography (GC), Flash column chromatography, Column chromatography, Ion chromatography, Gel-filtration chromatography or Size-exclusion chromatography, Pyrolysis gas chromatography, Countercurrent chromatography.