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Laboratory work is considered important for student achievements in science education. Biopharmaceutics and Pharmacokinetics subject has become very important course curriculum in pharmacy of different university of India and abroad. As a teacher, we have felt that one practical or experimental book of biopharmaceutics and pharmacokinetics is very essential for B.Pharmacy and M.Pharmacy students. It’s great pleasure that presenting my book entitled “Hand Book of Practical Biopharmaceutics and Pharmacokinetics” cover the most experiments laid down in different universities. The book contains different experiments related to absorption, metabolism, excretion, protein binding, pharmacokinetics and bioavailability chapter. We have included very simple experiments which are performed in laboratory in very easy setup instead of experiments requiring sophisticated instruments with high cost. Teachers can also refer this book for conducting any experiments related to biopharmaceutics as it is written according to All India Council for Technical Education and different universities syllabus for B. Pharmacy courses. We hope this book is very helpful for students of B.Pharmacy and M.Pharmacy courses. We have made a humble attempt to write the experiment in a comprehensive manner with possible care to avoid mistakes.