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This book is designed to guide and orient students of S.P.Pune University, Pune First year B.Sc. Program in their Zoology Practical course. Here all the practical from semester I and semester II are covered in detail with supporting diagrams and appropriate text information. After extensive peer review system this manual is configured to cater the needs of students and demand of SPPune University CBCS syllabus. This book is divided in to four distinct parts as per the sub-topics such as Animal diversity I and animal ecology for the first term and Animal diversity II and Cell biology for the second term. At end, supporting chapters for the field visit is also provided with detail information. Colored images/ photographs and illustrations will help student to understand the subject in depth. Line drawing will definitely help student in writing journals for routine practical. This book will also serve as practical teaching aid for the demonstrator.